EDUCATION

• Northwestern University, Ph.D
• University of Illinois at Urbana-Champaign, B.S.
  

RESEARCH Project

Megakaryopoiesis is of major importance in physiology and pathophysiology; myelodysplastic syndromes are often accompanied by defective megakaryocyte (MK) development, decreased platelet counts, and may progress into leukemia. We feel that understanding the fundamental molecular mechanisms underlying megakaryocytic differentiation and polyploidization is an essential first step to discovering novel therapeutic targets and approaches for platelet disorders and diseases associated with megakaryopoiesis such as megakaryoblastic leukemia. Unfortunately, the transcriptional mechanisms governing MK development are poorly understood due to in vitro culturing difficulties and their relative paucity in vivo. Recognizing this void, my research focuses on understanding the molecular mechanisms involved in megakaryocyte maturation and function. To accomplish this, genome-scale microarray analyses identify potential mediators of megakaryopoiesis, which are in turn analyzed by various cellular and molecular biological approaches. In this way, microarray analysis guides my research into the fundamental molecular mechanisms underlying megakaryocytic differentiation and polyploidization.

ADVISOR
E. Terry Papoutsakis, University of Delaware

SELECTED PUBLICATIONS

1. Petersen CE, Lindsey SC, Dudgeon DM, and Pertell RA. (1998) The Effect of Seed Predation on Pod Abortion by the Prairie Legume, Baptisia leucophaea. Transactions of the Illinois State Academy of Science 91:47-52.
   
2. Lindsey S, Zhu C, Lu YF, Eklund EA. (2005) HoxA10 Represses Transcription of the Gene Encoding p67PHOX in Phagocytic Cells. Journal of Immunology 175:5269-5279.
3. Huang W, Saberwal G, Horvath E, Zhu C, Lindsey S, Eklund EA. (2006) Leukemia-Associated, Constitutively Active Mutants of SHP2 Protein Tyrosine Phosphatase Inhibit NF1 Transcriptional Activation by the Interferon Consensus Sequence Binding Protein. Molecular and Cellular Biology 26:6311-6332.
4. Lindsey S, Huang W, Wang H, Horvath E, Zhu C, Eklund EA. (2007) Activation of SHP2 Protein Tyrosine Phosphatase Increases HoxA10-induced Repression of the Genes Encoding gp91PHOX and p67PHOX. Journal of Biological Chemistry 282:2237-2249.
5. Lindsey S*, Zhu C*, Konieczna I, Eklund EA. (2008) Constitutive Activation of SHP2 Protein Tyrosine Phosphatase Inhibits ICSBP-Induced Transcription of the Gene Encoding gp91PHOX During Myeloid Differentiation. Journal of Luekocyte Biology (In Press).
6. Konieczna I, Horvath E, Wang H, Lindsey S, Saberwal G, Bei L, Platanias L, Huang W, Eklund EA. (2008) Constitutive activation of SHP2 cooperates with ICSBP-deficiency to accelerate progression to acute myeloid leukemia. Journal of Clinical Investigation (In Press).
   
7. Lindsey S, Wang H, Konieczna I, Bei L, Horvath E, Eklund EA. (2008) Constitutively active SHP2 cooperates with HoxA10-overexpression for acute myeloid leukemia in a murine model. Blood (Under Revision).
8. Fuhrken P, Apostolidis P, Lindsey S, Miller WM, Papoutsakis ET. (2008) Tumor suppressor protein p53 regulates megakaryocytic endomitosis and apoptosis. Journal of Biological Chemistry (Under Revision).

RESEARCH SUMMARY KEYWORDS

• Hematopoiesis
• Megakaryocyte