54. Kunas, K.T., and Papoutsakis, E.T., "The protective effect of serum against hydrodynamic damage of hybridoma cells in agitated and surface aerated bioreactors", J. Biotechnol. 15: 57-70 (1990).
(Download PDF)
55. Kunas, K.T., and Papoutsakis, E.T., "Increasing serum concentrations decrease cells death and allow growth of hybridoma cells at higher agitation rates", Biotechnol. Lett. 11: 525-530 (1989).(Download PDF)
56. Cherry, R.S. and Papoutsakis, E.T., "Understanding and controlling injury of animal cells in bioreactors", in Animal Cell Biotechnology (R.E. Spier and J.B. Griffiths, eds.), Vol. 4. Academic Press. Chp. 3: pp. 72-121 (1990).
57. Kunas, K.T., and Papoutsakis E.T., "Damage mechanisms of suspended animal cells in agitated bioreactors with and without bubble entrainment", Biotechnol. Bioeng. 36: 476-483 (1990).(Download PDF)
58. Papoutsakis, E.T., "Developments in product recovery and purification", Chapter 13 in "Recombinant DNA Technology and Applications", A. Prokop, R. Bajpai, C. S. Ho, eds, pp. 357-412; McGraw-Hill, (1991).
59. Petersen, J.F., McIntire, L.V. and Papoutsakis, E.T., "Shear sensitivity of freely suspended animal cells in batch, fedbatch, and continuous cultures", Biotechnol. Progr. 6: 114-120 (1990).
60. Cary, J.W., Petersen, D.J., Papoutsakis, E.T., and G.N. Bennett, "Cloning and expression of Clostridium acetobutylicum ATCC 824 coenzyme A: acetate/butyrate: coenzyme A-transferase in Escherichia coli", Appl. Environ. Microbiol. 56, 1576-1583 (1990).(Download PDF)
61. Huesemann, M.H.W. and Papoutsakis, E.T., "Effects of propionate and acetate additions on solvent production in batch cultures of Clostridium acetobutylicum", Appl. Environ. Microbiol. 56:1497-1500 (1990).(Download PDF)
62. Papoutsakis, E.T., Petersen, J.F. and McIntire, L.V., "Cytoskeletal microfilament network and energy metabolism affect ability of animal cells to resist shear injury", in Production of Biologicals From Animal Cells In Culture (Proc. of the 10th ESACT Meeting, Avignon, France, May 1990) (Spier, R.E., Griffiths J. B., and Meigner, B., eds.). Butterworths, England. pp. 229-234 (1991).
63. Michaels, J. D., Petersen, J.F., McIntire, L.V. and Papoutsakis, E.T., "Protection mechanisms of freely suspended animal cells (CRL 8018) from fluid-mechanical injury. Viscometric and bioreactor studies using serum, Pluronic F68 and polyethylene glycol", Biotechnol. Bioeng. 38: 169-180 (1991).(Download PDF)
64. Michaels, J. D. and Papoutsakis, E.T., "Polyvinyl alcohol and polyethylene glycol as protectants against fluid-mechanical injury of freely suspended animal cells (CRL 8018)", J. Biotechnol. 19: 241-258 (1991).(Download PDF)
65. Lakhotia, S. and Papoutsakis, E.T., "Agitation induced cell injury in microcarrier cultures. The protective effect of viscosity is agitation-intensity dependent: Experiments and theory", Biotechnol. Bioeng. 39: 95-107 (1992). (Download PDF)
66. Papoutsakis, E.T., "Fluid-mechanical damage of animal cells in bioreactors (A review)", Trends in Biotechnol. 9: 427-437 (1991). (Download PDF)
67. Papoutsakis, E.T., "Media additives for protecting animal cells against agitation and aeration damage in bioreactors (A review)", Trends in Biotechnol. 9: 316-324 (1991).(Download PDF)
68. Petersen, D.J., Welch, R.W., Walter, K.A., Mermelstein, L.D., Papoutsakis, E.T., Rudolph, F.B., and Bennett, G.N., "Cloning of an NADH-dependent Butanol Dehydrogenase Gene from Clostridium acetobutylicum", Ann. N.Y. Acad. Sci. 646, 94-98 (1991).
69. Koller, M.R., Bender, J.G., Miller, W.M. and Papoutsakis, E.T., "Reduced oxygen tension increases hematopoiesis in long-term culture of human and progenitor cells from cord blood and bone marrow", Exper. Hematol. 20: 264-270 (1992).
70. Mermelstein, L.D., Welker, N.E., Bennett, G.N. and Papoutsakis, E.T., "Expression of Cloned Homologous Fermentative Genes in Clostridium acetobutylicum ATCC 824", Bio/Technol. 10: 190-195 (1992).
71. O'Connor, K.C. and Papoutsakis, E.T., "Agitation effects on microcarrier and suspension CHO cells", Biotechnol. Techn. 6: 323-328 (1992).(Download PDF)
72. Koller, M.R., Bender, J.G., Miller, W.M. and Papoutsakis, E.T., "Effects of synergistic cytokine combinations, low oxygen, and irradiated stroma on the expansion of human cord blood progenitors", Blood. 80: 403-411 (1992).(Download PDF)
73. Lakhotia, S., Bauer, K.D. and Papoutsakis, E.T., "Damaging agitation intensities increase DNA synthesis rate and alter cell cycle phase distributions of CHO cells", Biotechnol. Bioeng. 40: 978-990 (1992).(Download PDF)
74. Michaels, J.D., Kunas K.T. and Papoutsakis, E.T., "Fluid-mechanical Damage of freely-suspended cells in agitated bioreactors: Effects of dextran, derivatized celluloses and polyvinyl alcohol", Chem. Eng. Communic. 118: 341-360 (1992).
75. Lee, S.Y., Bennett. G.N. and Papoutsakis, E.T., "Construction of E. coli-Clostridium acetobutylicum shuttle vectors and transformation of C. acetobutylicum strains", Biotechnol. Lett. 14: 427-432 (1992).(Download PDF)
76. Lee, S.Y., Mermelstein, L.D., Bennett, G.N. and Papoutsakis, E.T., "Vector construction, transformation and gene amplification in Clostridium acetobutylicum ATCC 824", Ann. N.Y. Acad. Sci. 665: 39-51 (1992).
77. Koller, M.R., Bender, J.G., Papoutsakis, E.T. and Miller, W.M., "Beneficial effects of reduced oxygen tension and perfusion in long-term hematopoietic cultures", Ann. N.Y. Acad. Sci. 665: 105-116 (1992).
78. Borys, M.C. and Papoutsakis, E.T., "Formation of bridges, and large cellular clumps in CHO-cell microcarrier cultures: Effects of agitation, dimethyl sulfoxide and calf serum", Cytotechnol. 8: 237-248 (1992).(Download PDF)
79. Walter, K.A., Bennett, G.N. and Papoutsakis, E.T., "Molecular characterization of two Clostridium acetobutylicum ATCC 824 butanol dehydrogenase isozyme genes" J. Bacteriol. 174: 7149-7158 (1992).(Download PDF)
80. Koller, M.R., Bender, J.G., Miller, W.M. and Papoutsakis, E.T., "Expansion of primitive human hematopoietic progenitors in a perfusion bioreactor system with IL-3, IL-6 and stem-cell factor", Bio/Technol. 11: 358-363 (1993).(Download PDF)
81. Mermelstein, L.D. and Papoutsakis, E.T., "In vivo methylation in Escherichia coli by the Bacillus subtilis phage φ3T I Methyltransferase to protect plasmids from restriction upon transformation of Clostridium acetobuttylicum ATCC 824", Appl. Environ. Microbiol. 59: 1077-1081 (1993).(Download PDF)
82. Lakhotia, S., Bauer, K.D. and Papoutsakis, E.T., "Fluid-mechanical forces in agitated bioreactors reduce the CD13 and CD33 surface content of HL60 cells", Biotechnol. Bioeng. 41: 868-877 (1993).(Download PDF)
83. Borys, M.C., Linzer, D.I.H., and Papoutsakis, E.T., "Culture pH affects expression rates and glycosylation of recombinant mouse placental lactogen proteins by Chinese Hamster Ovary (CHO) cells", Bio/Technol. 11: 720-724 (1993).(Download PDF)
84. Lee, S.Y., Mermelstein, L.D., Bennett, G.N., and Papoutsakis, E.T. "Determination of plasmid copy number and stability in Clostridium acetobutylicum ATCC 824", FEMS Microbiol. Lett. 108: 319-324 (1993).
85. Papoutsakis, E.T. and Michaels, J.D., "Physical forces in mammalian cell bioreactors", in Physical Forces and the Mammalian Cells (J. Frangos, ed.). Academic Press, New York. Chp. 10: pp. 291-345 (1993).
86. Papoutsakis, E.T. and Bennett, G.N., "Cloning, structure, and expression of acid and solvent pathway genes of Clostridium acetobutylicum", Chapter 8 in: Clostridia and Biotechnology (Woods, D. R., ed.), pp. 157-199, Butterworth-Heinemann, Stoneham, MA (1993).
87. Borys, M.C. and Papoutsakis, E.T., "‘Oxygenation', Module 8.D:1 of Part 8D: Biochemistry of Cells in Culture. Environmental Factors", in Protocols in Cell and Tissue Culture (J. B. Griffiths, Doyle, A., and Newell, D. G., eds.). Wiley, Chichester, UK. pp. 8D1.1-8D.1.13 (1993).
88. Borys, M.C. and Papoutsakis, E.T., "‘Mixing', Module 8.D:2 of Part 8D: Biochemistry of Cells in Culture. Environmental Factors", in Protocols in Cell and Tissue Culture (J. B. Griffiths, Doyle, A., and Newell, D.G., eds.), Wiley, Chichester, UK. pp. 8D:2.1-8D.2.8 (1993).
89. Papoutsakis, E.T., "‘Mechanical Protection, Module 8.D:3 of Part 8D: Biochemistry of Cells in Culture. Environmental Factors", in Protocols in Cell and Tissue Culture (J. B. Griffiths, Doyle, A., and Newell, D.G., eds.), Wiley, Chichester, UK. pp. 8D:3.1-8D.3.8 (1993).
90. Mermelstein, L.D., Bennett, G.N. and Papoutsakis, E.T., "Amplification of homologous fermentative genes in Clostridium acetobutylicum ATCC 824", In: Bioproducts and Bioprocesses: Third Conference to Promote Japan/US Joint Projects and Cooperation in Biotechnology" (Tanner, R.D., ed.), pp. 317-343, Springer Verlag, New York (1993).
91. Mermelstein, L.D., Papoutsakis, E.T., Petersen, D.J. and Bennett, G.N., "Metabolic engineering of Clostridium acetobutylicum for increased solvent production by enhancement of acetone formation enzyme activities using a synthetic acetone operon" Biotechnol. Bioeng. 42: 1053-1060 (1993).(Download PDF)
92. Walter, K.A., Nair, R.V., Cary, J.W., Bennett, G.N., and Papoutsakis, E.T., "Sequence and arrangement of two genes encoding enzymes of the butyrate formation pathway of Clostridium acetobutylicum ATCC 824", Gene 134: 107-111 (1993).(Download PDF)
93. Mermelstein, L.D., and Papoutsakis, E.T., "Evaluation of macrolide and lincosamide antibiotics for plasmid maintenance in low pH Clostridium acetobutylicum ATCC 824 fermentations", FEMS Microbiol. Lett. 113: 71-75 (1993).
94. Walter, K.A., Mermelstein, L.D., and Papoutsakis, E.T., "Fermentations of recombinant Clostridium acetobutylicum with increased dosages of butyrate formation genes" Ann. N.Y. Acad. Sci. 721: 69-72 (1994).
95. Mermelstein, L.D., Welker, N.E., Petersen, D.J., Bennett, G.N., and Papoutsakis, E.T, "Genetic and metabolic engineering of Clostridium acetobutylicum ATCC 824", Ann. N.Y. Acad. Sci. 721: 54-68 (1994).
96. Nair, R.V., Bennett, G.N., and Papoutsakis, E.T., "Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824", J. Bacteriol. 176: 871-885 (1994).(Download PDF)
97. Michaels, J.D., Wasan, D. and Papoutsakis, E.T., "Dynamic interfacial tension and rheological properties of cell culture media with shear protectants", in Animal Cell Technology: Products of Today, Prospects for Tomorrow (R. E. Spier, J. B. Griffiths, W. Berthold, eds.), Butterworth-Heinemann. pp. 389-391 (1994).
98. Borys, M.C., Linzer, D.I.H. and Papoutsakis, E.T., "Culture pH and ammonia affect expression rates and glycosylation of recombinant mouse placental lactogen proteins by CHO cells", in Animal Cell Technology: Products of Today, Prospects for Tomorrow (R. E. Spier, J. B. Griffiths, W. Berthold, eds.), Butterworth-Heinemann. pp. 658-660 (1994).
99. Borys, M.C., Linzer, D.I.H. and Papoutsakis, E.T., "Ammonia affects the glycosylation patterns of recombinant mouse placental lactogen-1 (mPL-I) by Chinese Hamster Ovary (CHO) Cells", Biotechnol. Bioeng. 43: 505-514 (1994).(Download PDF)
100. Sandstrom, C.E., Miller, W.M. and Papoutsakis, E T., "Review: serum-free media for cultures of primitive and mature hematopoietic cells", Biotechnol. Bioeng. 43: 706-733 (1994).(Download PDF)
101. Borys, M.C., Linzer, D.I.H. and Papoutsakis, E.T., "Cell aggregation in a CHO microcarrier culture affects expression rates and N-linked glycosylation of recombinant mouse placenta lactogen-1 (mPL-1)", Ann. N. Y. Acad. Sci. 745: 360-371 (1994).
102. Koller, M.R. and Papoutsakis, E.T., "Cell adhesion in animal cell culture: physiological and fluid-mechanical implications", Chapter in "Cell Adhesion: Fundamentals and Biotechnological Applications" (M. Hjortso, J. Roos, eds), Bioprocess Technology Series, No. 20. Marcel Dekker, New York. 20: pp. 61-110 (1995).
103. Nair, R.V. and Papoutsakis, E.T., "Expression of plasmid encoded aad in Clostridium acetobutylicum M5 restores vigorous butanol production", J. Bacteriol. 176: 5843-5846 (1994).(Download PDF)
104. Walter, K.A., Mermelstein, L.D. and Papoutsakis, E.T., "Host-plasmid interactions in recombinant strains of Clostridium acetobutylicum ATCC 824", FEMS Microbiol. Lett., 123: 335-342 (1994).
105. Michaels, J.D., Nowak, J.E., Mallik, A.K., Koczo, K., Wasan, D.T., and Papoutsakis, E. T., "Analysis of cell-to-bubble attachment in sparged bioreactors in the presence of cell protecting additives against shear", Biotechnol. Bioeng. 47: 407-419 (1995).(Download PDF)
106. Michaels, J. D., Nowak, J.E., Mallik, A.K., Koczo, K., Wasan, D.T., and Papoutsakis, E.T., "Interfacial properties of cell culture media with cell protecting additives against shear", Biotechnol. Bioeng. 47: 420-430 (1995).(Download PDF)
107. Sandstrom, C.E., Bender, J.G., Papoutsakis, E.T., and Miller, W.M., "Effects of CD34+ cell selection and perfusion on Ex vivo expansion of peripheral blood mononuclear cells", Blood. 86: 958-970 (1995).(Download PDF)
108. Papoutsakis, E.T., McAdams, T.A., Sandstrom, C.E., Miller, W.M., and Bender, J.G. "Ex vivo Expansion of Primitive Hematopoietic Cells for Cellular Therapies: An Overview", Cytotechnology. 18: 133-146 (1995).(Download PDF)
109. Sandstrom, C.E., Bender, J.G., Miller, W.M., and Papoutsakis, E.T. "Development of novel perfusion chamber to retain nonadherenT-cells and its use for comparison of human "mobilized" peripheral-blood cell cultures with and without irradiated bone marrow stroma", Biotechnol. Bioeng. 50: 493-504 (1996).
110. Collins, P.C, Papoutsakis, E. T., & Miller, W.M. "Ex-vivo systems for hematopoietic cell therapies", Current Opinion in Biotechnology. 7: 223-230 (1996).
111. Stim-Herndon, K.P., Nair, R., Papoutsakis, E.T., and Bennett, G. N., "Analysis of degenerate variants of Clostridium acetobutylicum ATCC 824", Anaerobe, 2: 11-18 (1996).(Download PDF)
112. Michaels, J.D., Mallik, A.K., and Papoutsakis, E.T., "Sparging and agitation induced injury of cultured animal cells: Do bubble coalescence and breakup in the bulk liquid injure cells?", Biotechnol. Bioeng. 51: 399-409 (1996).
113. McAdams, T.A., Miller, W.M., and Papoutsakis, E.T., "Hematopoietic cell culture therapies: I. Cell culture considerations", Trends in Biotechnol. 14: 341-349 (September 1996).
114. McAdams, T.A., Winter, J. A., Miller, W.M., and Papoutsakis, E.T., "Hematopoietic cell culture therapies: II. Clinical aspects", Trends in Biotechnol. 14: 388-396 (October 1996).
115. Green, E.M., Boynton, Z.L., Harris, L.M., Rudolph, F.B., Papoutsakis, E. T. and Bennett, G.N., "Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824", Microbiology, 142: 2079-2986 (1996).
116. Sandstrom, C.E., Collins, P.C., McAdams, T.A., Bender, J.G., Papoutsakis, E.T., and Miller, W.M., "Comparison of serum-deprived media for the Ex vivo expansion of hematopoietic progenitor cells from cord blood and peripheral blood mononuclear cells", J. Hematotherapy. 5: 461-473 (1996).
117. LaIauppa, J.A., Papoutsakis, E.T., and Miller, W.M. "Evaluation of cytokines for the expansion of megakaryocyte and granulocyte lineages", Stem Cells. 15: 198-206 (1997).(Download PDF)
118. LaIuppa, J.A., Papoutsakis, E.T., and Miller, W.M., "Ex vivo expansion of hematopoietic stem and progenitor cells for transplantation", in Blood Stem Cell Transplantation (J. Winter, Editor), Kluwer, New York. Chp. 8: pp.159-186 (1997).
119. McAdams, T.A., Miller, W.M., and Papoutsakis, E.T. "Effects of culture pH on the expansion and differentiation of hematopoietic progenitors from peripheral and cord blood", Br. J. Haematol. 97: 889-895 (1997).
120. Collins, P. C., Wong, C-K., Papoutsakis, E. T., and Miller, W. M., "Real time method for determining the colony-forming cell content of human hematopoietic cell cultures", Biotechnol. Bioeng. 55: 693-700 (1997).(Download PDF)
121. LaIauppa, J.A., McAdams, T.A., Papoutsakis, E.T., and Miller, W.M. "Culture materials affect Ex vivo expansion of hematopoietic progenitor cells", J. Biomed. Mat. Res. 36: 347-359 (1997).(Download PDF)
122. Cornillot, E., Nair, R., Papoutsakis, E.T., & Soucaille, P. "The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to strain degeneration", J. Bacteriol. 179: 5442-5447 (1997).(Download PDF)
123. Bermejo, L.L., Welker, N.E and Papoutsakis, E.T. "Heterologous expression of Clostridium acetobutylicum ATCC824 genes in Escherichia coli for acetone production and acetate detoxification", Appl. Environ. Microbiol., 64: 1079-1085 (1998).(Download PDF)
124. Collins, P. C., Miller, W. M., and Papoutsakis, E. T., "Stirred culture of peripheral and cord-blood hematopoietic cells offers advantages over traditional static systems for clinically relevant applications", Biotechnol. Bioeng. 59: 534-543 (1998).(Download PDF)
125. Collins, P. C., Nielsen, L. K., Patel, S. D., Papoutsakis, E. T., and Miller, W. M., "Characterization of hematopoietic cell expansion, oxygen uptake and glycolysis in a controlled, stirred-tank bioreactor system", Biotechnol. Progr. 14: 466-472 (1998).(Download PDF)
126. LaIauppa, J.A., Papoutsakis, E.T., and Miller, W.M. "Oxygen tension alters the effects of cytokines on the megakaryocyte, erythrocyte and granulocyte lineages", Exp. Hematol. 26: 835-843 (1998).
127. Nielsen, L. K., Papoutsakis, E. T., and Miller, W. M., "Modeling Ex vivo hematopoiesis using chemical engineering metaphors", Chem. Eng. Sci. 53: 1913-1925 (1998).(Download PDF)
128. McDowell, C. L., and Papoutsakis, E T. "Decreasing extracellular pH increases CD13 receptor surface content and alters the metabolism of HL60 cells cultured in stirred tank reactors"", Biotechnol. Progr., 14: 567-572 (1998).(Download PDF)
129. McDowell, C. L., and Papoutsakis, E T. "Increased agitation intensity increases CD13 receptor surface content and mRNA levels, and alters the metabolism of HL60 cells cultured in a stirred tank bioreactor", Biotechnol. Bioeng. 60: 239-250 (1998).(Download PDF)
130. McDowell, C. L., Carver R., T., and Papoutsakis, E T. "Effects of Methocel A15LV, polyethylene glycol, and polivinyl alcohol on CD13 and CD33 receptor surface content and metabolism of HL60 cells cultured in stirred tank bioreactors", Biotechnol. Bioeng. 60: 251-258 (1998).(Download PDF)
131. McDowell, C. L., and Papoutsakis, E T. "Serum increases the CD13 receptor expression, reduces the transduction of fluid-mechanical forces, and alters the metabolism of HL60 cells cultured in agitated bioreactors", Biotechnol. Bioeng. 60: 259-268 (1998).(Download PDF)
132. Collins, P.C., Patel, S.D., Miller, W. M., and Papoutsakis, E. T. "Initiation, maintenance, and quantification of human hematopoietic cell cultures", in Methods in Molecular Medicine, Vol.18: Tissue Engineering Methods (J. R. Morgan, M. L. Yarmush, Eds), Human Press, Totowa, NJ. Chp. 22: pp. 271-292 (1999).
133. Horner, M, Miller, W.M., Ottino, J.M., and Papoutsakis, E.T., "Transport in a grooved perfusion flat-bed bioreactor for cell therapy applications", Biotechnol. Progr. 14: 689-699 (1998).(Download PDF)
134. McAdams, T.A., Miller, W.M., and Papoutsakis, E.T., "pH is a potent modulator of erythroid differentiation", Br. J. Haematol. 103: 317-325 (1998).
135. Nair, R., Green E., Bennett, G. N. and Papoutsakis, E T. "Regulation of the sol locus genes for butanol and acetone production in Clostridium acetobutylicum ATCC 824 by a putative transcriptional repressor", J. Bacteriol. 181: 319-330 (1999).(Download PDF)
136. Desai, R. P. and Papoutsakis, E. T., "Antisense RNA strategies for the metabolic engineering of Clostridium acetobutylicum", Appl. Environ. Microbiol. 65: 936-945 (1999).(Download PDF)
137. Nielsen, L. K., Bender, J. G., Miller, W.M., and Papoutsakis, E. T., "Population balance model of in vivo neutrophil formation following bone marrow rescue therapy", Cytotechnology. 28 (1/3): 157-162 (1998).(Download PDF)
138. Desai, R., Nielsen, L. K., and Papoutsakis, E. T. "Stoichiometric modeling of Clostridium acetobutylicum fermentations with nonlinear constraints", J. Biotechnol. 71: 191-205 (1999).(Download PDF)
139. Mostafa, S. S, Hevehan, D. L., McAdams, T.A., Papoutsakis, E. T. and Miller, W. M., "Hematopoietic cells for cellular and gene therapy: I. Basic assay techniques", in Animal Cell Biotechnology: Methods and Protocols (N. Jenkins, Ed.), Humana Press. Chapter 17: pp. 211-227 (1999).(Download PDF)
140. McAdams, T.A., Papoutsakis, E. T. and Miller, W. M., "Hematopoietic cells for cellular and gene therapy: II. Expansion Protocols", in Animal Cell Biotechnology: Methods and Protocols (N. Jenkins, Ed.), Human Press. Chapter 18: pp. 229-238 (1999).(Download PDF)
141. Collins, P. C., Papoutsakis, E. T., Patel, S. and Miller, W. M., "Nuclei size distribution as a predictor of hematopoietic cell proliferation", Cytotherapy. 1: 99-110 (1999).
142. Papoutsakis, E. T., and Bennett, G. N. "Metabolic Engineering of Clostridium acetobutylicum", pp. 253-279, Chapter 11 in "Metabolic Engineering" (S.Y. Lee and E. T. Papoutsakis, Eds), Marcel Dekker, 1999.
143. Lee, S. Y. and Papoutsakis, E. T. "The challenges and promise of metabolic engineering", pp.1-12, Chapter 1 in "Metabolic Engineering" (S.Y. Lee and E. T. Papoutsakis, Eds), Marcel Dekker, 1999.
144. Guo, M. H., Miller, W. M., Papoutsakis, E. T., Patel, S., James, C., Goolsby, C., and Winter J. N., "Ex vivo expansion of CFU-GM and BFU-E in unselected peripheral blood mononuclear cell cultures with Flt3L in enhanced by autologous plasma", Cytotherapy. 1: 183-194 (1999).
145. Tummala, S. B., Welker, N. E., and Papoutsakis, E. T., "Development and characterization of a gene-expression reporter system for Clostridium acetobutylicum ATCC 824", Appl. Environ. Microbiol. 65: 3793-3799 (1999).(Download PDF)
146. Desai, R. P., Harris, L. M., Welker, N. E., Papoutsakis, E. T. "Metabolic flux analysis elucidates the importance of the acid-formation pathway in regulating solvent production by Clostridium acetobutylicum", Metabolic Eng. 1: 206-213 (1999).(Download PDF)