We use the New Brunswick Scientific BioFlo 310® Benchtop Fermenter System for large-scale fermentations. Generally, these fermenters are used when a large cell population is need, like for transcriptional studies using microarrays, or to test the solvent production of engineered strains under controlled conditions (e.g. pH and agitation). The parameters we use for typical, anaerobic clostridia fermentations are:
Major components and their functions and part numbers:
We have four 5L vessels, four control towers, and two controllers. Typically, one controller is linked to two vessels/control towers, but one controller can also be linked to three or four vessels/control towers. Our controllers and control towers can regulate the agitation rate, temperature, and pH of the culture. Aeration rate is controlled by external rotometers.
We have two recirculating water chillers with each chiller hooked up to two control towers. The chillers both ensure the water used to cool the fermenters is around 25°C and recycle the discharged water from the control tower.
Note: For Further details contact Shawn Jones or Dinesh Indurthi .